Caspase-2 Recombinant Rabbit Monoclonal Antibody [SR44-01]

About this product

Caspase-2 (Nedd2, ICH-1) is an aspartate-specific cysteine protease that is activated in response to various apoptotic stimuli. Caspase-2 is unique among the caspases in that it has features of both upstream caspases (long prodomain) and downstream caspases (DEXD substrate specificity). Caspase-2 is highly expressed in the brain during development, and is expressed at low levels in adult tissue. Specifically, caspase-2 localizes to the mitochondria, the Golgi, the cytoplasm, and the nucleus. Caspase-2 exists as two isoforms, caspase-2L and caspase-2S, which are produced by alternative splicing and differ in their N and C-termini. Caspase-2L acts as a positive regulator of apoptosis, whereas caspase-2S functions as a negative regulator of apoptosis. Following apoptotic stimuli, the caspase-2L precursor undergoes cleavage at Asp-153 to produce a fragment (p30). The p30 fragment undergoes further cleavage to generate a fragment containing amino acids 153-308 (p18) and a fragment containing amino acids 317-435 (p13 or p14). As apoptosis progresses, the p13 (p14) fragment can undergo further processing to yield a fragment containing amino acids 331-435 (p12).

Specifications

Catalog number

ET1602-30

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Caspase2 CASP 2 antibody CASP-2 antibody Casp2 antibody CASP2_HUMAN antibody Caspase 2 antibody Caspase 2 apoptosis related cysteine peptidase antibody Caspase-2 subunit p12 antibody Caspase2 antibody ICH 1 antibody ICH 1 protease antibody ICH 1L antibody ICH1 antibody ICH1 protease antibody ICH1L antibody NEDD-2 antibody NEDD2 antibody Neural precursor cell expressed developmentally down-regulated protein 2 antibody PPP1R57 antibody Protease ICH-1 antibody Protein phosphatase 1 regulatory subunit 57 antibody

Abbreviation

CASP-2, NEDD-2

Swiss Prot

P42575 Human, P29594 Mouse

Cellular Locus

Cytoplasm, Nucleus, Membrane.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein within mouse Caspase-2 aa 294-452 / 452.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

293 cell lysate, Jurkat cell lysate, HUVEC cell lysate, human lung carcinoma tissue lysate, Hela, A549, human kidney tissue, mouse kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;IHC-P: 1:50 ;FC: 1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 51 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes