Prohibitin Recombinant Rabbit Monoclonal Antibody [SR46-02]

About this product

Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation. Prohibitin mRNA and protein levels are high in G1, decline during the S phase, rise again in G2 and decline in M phase, which suggests that prohibitin controls the cell cycle by using both transcriptional and posttranslational mechanisms. Prohibitin is also a potential tumor suppressor protein that binds to retinoblastoma (Rb) and subsequently inhibits the activity of E2F family members in response to specific signaling cascades. Prohibitin 2 is a repressor of estrogen receptor activity, and is required for somatic and germline differentiation in the larval gonad during embryonic development. Mutations in the Prohibitin genes are correlated with breast cancer development and/or progressionin more than 80% of the cell lines analyzed.

Specifications

Catalog number

ET1602-31

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Epididymis luminal protein 215 antibody Epididymis secretory sperm binding protein Li 54e antibody HEL 215 antibody HEL S 54e antibody PHB antibody PHB_HUMAN antibody PHB1 antibody Prohibitin antibody

Abbreviation

PHB

Swiss Prot

P35232 Human, P67778 Mouse, P67779 Rat, Q7T1D8 Zebrafish

Cellular Locus

Cell membrane, Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Synthetic peptide within Human Prohibitin aa 147-196 / 272.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, IP, FC

Positive Control

HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate, hybrid fish (crucian-carp) liver tissue lysates, HepG2, PC-12, NIH/3T3, human liver carcinoma tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue, mouse stomach tissue, mouse heart tissue, human liver tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100-1:1,000 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 30 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes