Phospho-Rb (S807) Recombinant Rabbit Monoclonal Antibody [SR3-82]

About this product

Pediatric cancer retinoblastoma and the formation of other human tumors can be attributed to mutations in the retinoblastoma tumor suppressor gene (Rb). The Rb protein regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle at G1-S with transcriptional machinery. During G1, cyclin D-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser-807/ Ser 811 and Thr 821/Thr 826. Hypophosphorylated Rb represses the transcription of genes controlling the cell cycle through direct protein-protein interactions and through the recruitment of histone deacetylase.

Specifications

Catalog number

ET1602-36

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

OSRC antibody Osteosarcoma antibody p105-Rb antibody P105RB antibody PP105 antibody pp110 antibody PPP1R130 antibody pRb antibody Prepro retinoblastoma associated protein antibody Protein phosphatase 1 regulatory subunit 130 antibody Rb antibody RB_HUMAN antibody RB1 antibody RB1 gene antibody Retinoblastoma 1 antibody Retinoblastoma suspectibility protein antibody Retinoblastoma-associated protein antibody

Abbreviation

Rb

Swiss Prot

P06400 Human, P13405 Mouse, P33568 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser807 of Human Rb.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P

Positive Control

K-562 cell lysate, K-562, L929, C6, human tonsil tissue, human lung squamous cell carcinoma tissue, human spleen tissue, mouse spleen tissue, rat spleen tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:50-1:100 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 106 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes