Histone H4 (acetyl K5) Recombinant Rabbit Monoclonal Antibody [SR31-07]

About this product

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Specifications

Catalog number

ET1602-40

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Histone gene cluster 1, H4A antibody Histone gene cluster 2, H4 antibody dJ160A22.1 antibody dJ160A22.2 antibody dJ221C16.1 antibody dJ221C16.9 antibody FO108 antibody H4 antibody H4 histone family, member A antibody H4 histone family, member B antibody H4 histone family, member C antibody H4 histone family, member D antibody H4 histone family, member E antibody H4 histone family, member G antibody H4 histone family, member H antibody H4 histone family, member I antibody H4 histone family, member J antibody H4 histone family, member K antibody H4 histone family, member M antibody H4 histone family, member N antibody H4 histone, family 2 antibody H4/A antibody H4/B antibody H4/C antibody H4/D antibody H4/E antibody H4/G antibody H4/H antibody H4/I antibody H4/J antibody H4/K antibody H4/M antibody H4/N antibody H4/O antibody H4/p antibody H4_HUMAN antibody H4F2 antibody H4F2iii antibody H4F2iv antibody H4FA antibody H4FB antibody H4FC antibody H4FD antibody H4FE antibody H4FG antibody H4FH antibody H4FI antibody H4FJ antibody H4FK antibody HIST1 cluster, H4A antibody HIST1 cluster, H4B antibody HIST1 cluster, H4D antibody HIST2H4 antibody Hist4 cluster, H4 antibody Hist4h4 antibody histone 1, H4a antibody histone 1, H4c antibody histone 1, H4d antibody histone 1, H4f antibody histone 1, H4h antibody histone 1, H4i antibody histone 1, H4j antibody histone 1, H4k antibody histone 1, H4l antibody histone 2, H4a antibody histone 2, H4b antibody Histone 4 family, member M antibody histone 4, H4 antibody histone cluster 1, H4 antibody histone cluster 1, H4a antibody histone cluster 1, H4b antibody histone cluster 1, H4c antibody histone cluster 1, H4d antibody histone cluster 1, H4e antibody histone cluster 1, H4f antibody histone cluster 1, H4h antibody histone cluster 1, H4i antibody histone cluster 1, H4j antibody histone cluster 1, H4k antibody histone cluster 1, H4l antibody histone cluster 2, H4a antibody histone cluster 2, H4b antibody histone cluster 4, H4 antibody Histone family, member A antibody Histone family, member B antibody Histone family, member D antibody Histone family, member H antibody Histone family, member I antibody Histone family, member L antibody Histone gene cluster 1, H4 antibody Histone gene cluster 1, H4D antibody Histone gene cluster 1, H4E antibody Histone gene cluster 1, H4K antibody Histone gene cluster 4, H4 antibody Histone gene cluster 4, H4 histone antibody Histone H4 antibody histone IV, family 2 antibody H4K5ac antibody

Abbreviation

Histone H4 H4K5ac

Swiss Prot

P62805 Human, P62806 Mouse, P62804 Rat

Cellular Locus

Nucleus, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Histone H4 aa 1-50 / 103 (acetyl K5).

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, ChIP, CUT&Tag-seq

Positive Control

HeLa cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 400nM TSA for 18 hours cell lysate, C6 cell lysate, C6 treated with 1μM TSA for 18 hours cell lysate, HeLa, NIH/3T3, PC-12, human testis tissue, human colon tissue, human tonsil tissue, human colon carcinoma tissue, mouse testis tissue, mouse colon tissue, mouse brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:50-1:400 ;IHC-P: 1:200-1:1,000 ;IP: Use at an assay dependent concentration. ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 11 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes