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Product details

Histone H3 (mono+di+tri methyl K79) Recombinant Rabbit Monoclonal Antibody [SR42-06]

Catalog number ET1602-41

Price for ET1602-41

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Size / count 100 µL

About this product

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Specifications

Catalog number
ET1602-41
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
H3 histone family, member J antibody FLJ92264 antibody H3 histone family, member A antibody H3 histone family, member B antibody H3 histone family, member C antibody H3 histone family, member D antibody H3 histone family, member F antibody H3 histone family, member H antibody H3 histone family, member I antibody H3 histone family, member K antibody H3 histone family, member L antibody H3 histone, family 3A antibody H3.3A antibody H3/a antibody H3/b antibody H3/c antibody H3/d antibody h3/f antibody H3/h antibody H3/i antibody H3/j antibody H3/k antibody H3/l antibody H31_HUMAN antibody H3F1K antibody H3F3 antibody H3FA antibody H3FB antibody H3FC antibody H3FD antibody H3FF antibody H3FH antibody H3FI antibody H3FJ antibody H3FK antibody H3FL antibody HIST1H3A antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody Histone 1, H3a antibody Histone 1, H3b antibody Histone 1, H3c antibody Histone 1, H3d antibody Histone 1, H3e antibody Histone 1, H3f antibody Histone 1, H3g antibody Histone 1, H3h antibody Histone 1, H3i antibody Histone 1, H3j antibody Histone cluster 1, H3a antibody Histone cluster 1, H3b antibody Histone cluster 1, H3c antibody Histone cluster 1, H3d antibody Histone cluster 1, H3e antibody Histone cluster 1, H3f antibody Histone cluster 1, H3g antibody Histone cluster 1, H3i antibody Histone cluster 1, H3j antibody Histone H 3 antibody Histone H3.1 antibody Histone H3.2 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody Histone H3/m antibody Histone H3/o antibody H3K79me1me2me3 antibody
Abbreviation
Histone H3 H3K79me1me2me3
Swiss Prot
P68431 Human, P84243 Human, Q16695 Human, Q6NXT2 Human, Q71DI3 Human, P68433 Mouse, Q6LED0 Rat
Cellular Locus
Nucleus, Chromosome.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide within Human Histone H3 aa 56-105 / 136 (tri methyl K79) conjugated to Keyhole Limpet Haemocyanin (KLH).
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P, ChIP
Positive Control
Mouse testis tissue lysate, CRC cell lysate, rat muscle tissue, rat skin tissue, human colon carcinoma tissue, human skin tissue, mouse colon tissue, rat colon tissue.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:2,000 ;IHC-P: 1:1,000-1:5,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 15 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for Histone H3 (mono+di+tri methyl K79) Recombinant Rabbit Monoclonal Antibody [SR42-06]?

The catalog number for Histone H3 (mono+di+tri methyl K79) Recombinant Rabbit Monoclonal Antibody [SR42-06] is ET1602-41.

What sizes are available for Histone H3 (mono+di+tri methyl K79) Recombinant Rabbit Monoclonal Antibody [SR42-06]?

Histone H3 (mono+di+tri methyl K79) Recombinant Rabbit Monoclonal Antibody [SR42-06] is available in the following size(s): 100 µL.

Is Histone H3 (mono+di+tri methyl K79) Recombinant Rabbit Monoclonal Antibody [SR42-06] in stock?

Histone H3 (mono+di+tri methyl K79) Recombinant Rabbit Monoclonal Antibody [SR42-06] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Histone H3 (mono+di+tri methyl K79) Recombinant Rabbit Monoclonal Antibody [SR42-06] used for?

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form t

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

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