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Product details

Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86]

Catalog number ET1602-44

Price for ET1602-44

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Size / count 100 µL

About this product

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Specifications

Catalog number
ET1602-44
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
ec 1.13.12.7 antibody Firefly antibody Luciferase antibody Luciferin 4 monooxygenase antibody
Abbreviation
Luciferase
Swiss Prot
P08659 Firefly
Cellular Locus
Peroxisome.
Host
Rabbit
Species Reactivity
Firefly
Immunogen
Recombinant protein within Firefly Luciferase aa 132-243 / 550.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue
Positive Control
Luciferase recombinant protein.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 61 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86]?

The catalog number for Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86] is ET1602-44.

What sizes are available for Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86]?

Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86] is available in the following size(s): 100 µL.

Is Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86] in stock?

Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86] used for?

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, res

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

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