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Product details

Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04]

Catalog number ET1602-46

Price for ET1602-46

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Size / count 100 µL

About this product

Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors.

Specifications

Catalog number
ET1602-46
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
ECK4026 antibody JW3994 antibody Mal E antibody MalE antibody malJ antibody Maltodextrin binding protein antibody Maltose ABC transporter periplasmic protein antibody Maltose binding periplasmic protein antibody MMBP antibody Periplasmic maltose binding protein antibody
Abbreviation
malE
Swiss Prot
P0AEX9 EscherichiaColi, P0AEY0 EscherichiaColi
Host
Rabbit
Species Reactivity
Escherichia coli
Immunogen
Synthetic peptide within Escherichia coli MBP aa 331-380 / 396.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IP
Positive Control
Recombinant MBP-tag protein.
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:5,000 ;IP: 2-5 µg/ml.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 43 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04]?

The catalog number for Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04] is ET1602-46.

What sizes are available for Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04]?

Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04] is available in the following size(s): 100 µL.

Is Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04] in stock?

Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04] used for?

Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utiliz

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

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