Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06] — image 1 of 3

Product details

Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06]

Catalog number ET1603-14

Discontinued

Price for ET1603-14

Size / count 100 µL

This variant is discontinued and cannot be added to your cart.

About this product

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex. eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.

Specifications

Catalog number: ET1603-14
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: Yes
CAS Number: 9000-83-3
Product Name Alternative: EIF 2 alpha antibody EIF 2 antibody EIF 2A antibody EIF 2alpha antibody eIF-2-alpha antibody eIF-2A antibody EIF-2alpha antibody EIF2 alpha antibody EIF2 antibody EIF2A antibody EIF2S1 antibody Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody Eukaryotic translation initiation factor 2 subunit 1 alpha antibody Eukaryotic translation initiation factor 2 subunit 1 antibody Eukaryotic translation initiation factor 2 subunit alpha antibody IF2A_HUMAN antibody
Abbreviation: EIF2A, CDA02, MSTP004, MSTP089
Swiss Prot: P05198 Human, Q6ZWX6 Mouse, P68101 Rat
Cellular Locus: Stress granule, Cytoplasm.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser51 of Human eIF-2a.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Positive Control: HeLa treated with 50nM Calyculin A for 3 hours whole cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate, C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate, THP-1 cell lysate, C2C12 cell lysate, mouse spleen tissue, rat spleen tissue, human liver tissue, human pancreas tissue, mouse brain tissue, mouse placenta tissue, mouse pancreas tissue, human prostate carcinoma tissue, human breast carcinoma tissue, human colon carcinoma tissue, Hela.
Concentration: 1 mg/mL
Dilution: WB: 1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:500 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 36 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06]?

The catalog number for Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06] is ET1603-14.

Is Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06] in stock?

Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Phospho-EIF2S1 (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06] used for?

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.