Caspase-8 Recombinant Rabbit Monoclonal Antibody [SZ01-08]

About this product

Caspase-8 is a caspase protein, encoded by the CASP8 gene. It most likely acts upon caspase-3. CASP8 orthologs have been identified in numerous mammals for which complete genome data are available. These unique orthologs are also present in birds. The CASP8 gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Specifications

Catalog number

ET1603-16

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ALPS2B antibody Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody Apoptotic cysteine protease antibody Apoptotic protease Mch-5 antibody Apoptotic protease Mch5 antibody CAP4 antibody CASP-8 antibody CASP8 antibody CASP8_HUMAN antibody Caspase8 Caspase 8 antibody Caspase 8 apoptosis related cysteine peptidase antibody Caspase-8 subunit p10 antibody CED 3 antibody FADD Like ICE antibody FADD-homologous ICE/CED-3-like protease antibody FADD-like ICE antibody FLICE antibody FLJ17672 antibody ICE-like apoptotic protease 5 antibody MACH alpha 1/2/3 protein antibody MACH antibody MACH beta 1/2/3/4 protein antibody MCH5 antibody MGC78473 antibody MORT1 associated ced 3 homolog antibody MORT1-associated CED-3 homolog antibody OTTHUMP00000163717 antibody OTTHUMP00000163720 antibody OTTHUMP00000163724 antibody OTTHUMP00000163725 antibody OTTHUMP00000165062 antibody OTTHUMP00000165063 antibody OTTHUMP00000165064 antibody OTTHUMP00000206552 antibody OTTHUMP00000206582 antibody

Abbreviation

CASP-8, FLICE, MACH, CASP8

Swiss Prot

Q14790 Human

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human Caspase-8 aa 207-256 / 479.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Tissue, IHC-P

Positive Control

Jurkat cell lysate, HeLa cell lysate, THP-1 cell lysate, HepG2 cell lysate, 293T cell lysate, human tonsil tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 55 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes