NF-kB p105/p50 Recombinant Rabbit Monoclonal Antibody [SZ20-01]

About this product

Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFkB (p50 and p65) and the Drosophila maternal morphagen, dorsal. These proteins share sequence homology over a region of 300 amino acids at their NH2-terminus, the region that contains their DNA binding and dimerization domains. The DNA binding activity of NF B is activated and rapidly transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins have been described. These proteins, designated p105 and p100, are highly related but map on different chromosomes. The p105 (p110) precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated PdI, binds to p50 and regulates its activity.

Specifications

Catalog number

ET1603-18

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DKFZp686C01211 antibody DNA binding factor KBF1 antibody DNA binding factor KBF1 EBP1 antibody DNA-binding factor KBF1 antibody EBP 1 antibody EBP-1 antibody EBP1 antibody KBF1 antibody MGC54151 antibody NF kappa B antibody NF kappaB antibody NF kappabeta antibody NF kB1 antibody NFkappaB antibody NFKB 1 antibody NFKB p105 antibody NFKB p50 antibody Nfkb1 antibody NFKB1_HUMAN antibody Nuclear factor kappa B DNA binding subunit antibody Nuclear factor kappa-B, subunit 1 antibody Nuclear factor NF kappa B p105 subunit antibody Nuclear factor NF kappa B p50 subunit antibody Nuclear factor NF-kappa-B p50 subunit antibody Nuclear factor of kappa light chain gene enhancer in B cells 1 antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody p105 antibody p50 antibody p84/NF-kappa-B1 p98 antibody Transcription factor NFKB1 antibody

Abbreviation

NFKB1

Swiss Prot

P19838 Human, P25799 Mouse, Q63369 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human NFkB p105/p50 aa 330-370.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, ICC, FC, IF-Tissue

Positive Control

THP-1 cell lysate, Raji cell lysate, Daudi cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, Hela cell lysate, PC-12 cell lysate, human tonsil tissue, human spleen tissue, mouse bladder tissue, mouse prostate tissue.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IHC-P: 1:200-1:1,000 ;ICC: 1:100 ;FC: 1:1,000 ;IF-Tissue: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 105/50 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes