Phospho-Erk1 (T202)+Erk2 (T185) Recombinant Rabbit Monoclonal Antibody [SZ2-4]

About this product

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.

Specifications

Catalog number

ET1603-22

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ERK 1 antibody ERK 2 antibody ERK-1 antibody ERK-2 antibody ERK1 antibody erk1/2 antibody ERK2 antibody ERT1 antibody ERT2 antibody Extracellular signal regulated kinase 1 antibody Extracellular signal-regulated kinase 1 antibody Extracellular signal-regulated kinase 2 antibody HS44KDAP antibody HUMKER1A antibody Insulin-stimulated MAP2 kinase antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase 3 antibody MAP kinase isoform p42 antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 2 antibody MAPK 3 antibody Mapk1 antibody MAPK2 antibody MAPK3 antibody Microtubule-associated protein 2 kinase antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody Mitogen-activated protein kinase 3 antibody MK01_HUMAN antibody p38 antibody p40 antibody p41 antibody p41mapk antibody p42-MAPK antibody P42MAPK antibody p44-ERK1 antibody p44-MAPK antibody p44ERK1 antibody p44MAPK antibody PRKM 2 antibody PRKM1 antibody PRKM2 antibody PRKM3 antibody protein tyrosine kinase ERK2 antibody

Abbreviation

MAP kinase 3, MAPK 3, ERK-1, p44-MAPK, MAPK3, ERK1, PRKM3, MAP kinase 1, MAPK 1, ERK-2, p42-MAPK, MAP kinase 2, MAPK 2, MAPK1, ERK2, PRKM1, PRKM2

Swiss Prot

P27361 Human, P28482 Human, P63085 Mouse, Q63844 Mouse

Cellular Locus

Cytoplasm, Nucleus, Membrane, Cell junction.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr185 of Human Erk2.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC, IF-Tissue

Positive Control

Jurkat treated with 200ng/mL PMA for 35 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, C6 cell lysate, C6 treated with 200nM PMA for 30 minutes cell lysate, A549, NIH/3T3, MCF-7, human lung carcinoma tissue, human kidney tissue, human gallbladder tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100-1:500 ;IHC-P: 1:200-1:1,000 ;FC: 1:50-1:100 ;IF-Tissue:1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 43/41 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes