Phospho-c-Myc (T58 + S62) Recombinant Rabbit Monoclonal Antibody [SZ02-06]

About this product

c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.

Specifications

Catalog number

ET1603-24

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Avian myelocytomatosis viral oncogene homolog antibody bHLHe39 antibody c Myc antibody Class E basic helix-loop-helix protein 39 antibody MRTL antibody Myc antibody Myc protein antibody Myc proto oncogene protein antibody Myc proto-oncogene protein antibody myc related translation/localization regulatory factor antibody MYC_HUMAN antibody Myc2 antibody MYCC antibody Niard antibody Nird antibody Proto-oncogene c-Myc antibody Transcription factor p64 antibody v myc avian myelocytomatosis viral oncogene homolog antibody v myc myelocytomatosis viral oncogene homolog antibody

Abbreviation

bHLHe39, MYC

Swiss Prot

P01106 Human, P01108 Mouse

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of Human c-Myc.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IP, FC

Positive Control

HCT 116 cell lysate, HCT 116 treated with 25μM MG-132 for 4 hours cell lysate, EL4 cell lysate, EL4 treated with 25μM MG-132 for 4 hours cell lysate, K562.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 49 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes