Caspase-9 Recombinant Rabbit Monoclonal Antibody [SZ29-01]

About this product

Caspase-9 is an enzyme that in humans is encoded by the CASP9 gene. It is an initiator caspase, critical to the apoptotic pathway found in many tissues. Caspase-9 homologs have been identified in all mammals for which they are known to exist, such as Mus musculus and Pan troglodytes. Caspase-9 belongs to a family of caspases, cysteine-aspartic proteases involved in apoptosis and cytokine signalling. Apoptotic signals cause the release of cytochrome c from mitochondria and activation of apaf-1 (apoptosome), which then cleaves the pro-enzyme of caspase-9 into the active dimer form. Regulation of this enzyme occurs through phosphorylation by an allosteric inhibitor, inhibiting dimerization and inducing a conformational change. Correct caspase-9 function is required for apoptosis, leading to the normal development of the central nervous system. Caspase-9 has multiple additional cellular functions that are independent of its role in apoptosis. Nonapoptotic roles of caspase-9 include regulation of necroptosis, cellular differentiation, innate immune response, sensory neuron maturation, mitochondrial homeostasis, corticospinal circuit organization, and ischemic vascular injury.

Specifications

Catalog number

ET1603-27

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Caspase9 APAF-3 antibody APAF3 antibody Apoptosis related cysteine peptidase antibody Apoptotic protease Mch-6 antibody Apoptotic protease-activating factor 3 antibody CASP-9 antibody CASP9 antibody CASP9_HUMAN antibody Caspase 9 apoptosis related cysteine peptidase antibody Caspase 9 Dominant Negative antibody Caspase 9c antibody Caspase-9 antibody Caspase-9 subunit p10 antibody ICE LAP6 antibody ICE like apoptotic protease 6 antibody ICE-LAP6 antibody ICE-like apoptotic protease 6 antibody MCH6 antibody PPP1R56 antibody protein phosphatase 1, regulatory subunit 56 antibody RNCASP9 antibody

Abbreviation

CASP-9, APAF-3, ICE-LAP6, CASP9

Swiss Prot

P55211 Human, Q8C3Q9 Mouse

Cellular Locus

Mitochondrion, cytoplasm, cytosol, nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human Caspase-9 aa 289-338 / 416.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Positive Control

C2C12 cell lysate, Hela cell lysate, NIH/3T3, HepG2 cell, A549 cell, human tonsil tissue, human cervix carcinoma tissue, human colon tissue, human colon carcinoma tissue, mouse spleen tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:5,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration. ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 46 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes