Bcl-XL Recombinant Rabbit Monoclonal Antibody [SZ3-03]

About this product

It is a well-established concept in the field of apoptosis that relative amounts of pro- and anti-survival Bcl-2 family of proteins determine whether the cell will undergo cell death; if more Bcl-xL is present, then pores are non-permeable to pro-apoptotic molecules and the cell survives. However, if Bax and Bak become activated, and Bcl-xL is sequestered away by gatekeeper BH3-only factors (e.g. Bim) causing a pore to form, cytochrome c is released leading to initiation of caspase cascade and apoptotic events. While the exact signaling pathway of Bcl-xL is still not known, it is believed that Bcl-xL differs highly from Bcl-2 in their mechanism of inducing apoptosis. Bcl-xL is about ten times more functional than Bcl-2 when induced by the chemotherapy drug, Doxorubicin and can specifically bind to cytochrome C residues, preventing apoptosis. It can also prevent the formation of Apaf-1 and Caspase 9 complex by acting directly upon Apaf-1 rather than Caspase 9, as shown in nematode homologs.

Specifications

Catalog number

ET1603-28

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Apoptosis regulator Bcl X antibody Apoptosis regulator Bcl-X antibody Apoptosis regulator BclX antibody B cell lymphoma 2 like antibody B2CL1_HUMAN antibody Bcl 2 like 1 protein antibody Bcl X antibody Bcl xL antibody BCL XL/S antibody Bcl xS antibody Bcl-2-like protein 1 antibody Bcl2 Like 1 antibody Bcl2 related gene antibody Bcl2-L-1 antibody BCL2L antibody Bcl2l1 antibody BCLX antibody BclXL antibody BclXs antibody DKFZp781P2092 antibody PPP1R52 antibody Protein phosphatase 1 regulatory subunit 52 antibody

Abbreviation

Bcl2-L-1, BCL2L1

Swiss Prot

Q07817 Human, Q64373 Mouse, P53563 Rat

Cellular Locus

Mitochondrion inner membrane, Mitochondrion outer membrane, Mitochondrion matrix, Cytoplasmic vesicle, Cytoplasm, Nucleus membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Bcl-XL aa 37-86 / 233.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IP

Positive Control

K-562 cell lysate, Jurkat cell lysate, B16-F1 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Rat brain tissue lysate, K-562, human kidney tissue, mouse kidney tissue, rat kidney tissue, human colon carcinoma tissue, human breast carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 26 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes