Bax Recombinant Rabbit Monoclonal Antibody [SZ3-07]

About this product

In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. BAX is believed to interact with, and induce the opening of the mitochondrial voltage-dependent anion channel, VDAC. Alternatively, growing evidence also suggests that activated BAX and/or Bak form an oligomeric pore, MAC in the MOM (mitochondrial outer membrane). This results in the release of cytochrome c and other pro-apoptotic factors from the mitochondria, often referred to as mitochondrial outer membrane permeabilization, leading to activation of caspases. This defines a direct role for BAX in mitochondrial outer membrane permeabilization. BAX activation is stimulated by various abiotic factors, including heat, hydrogen peroxide, low or high pH, and mitochondrial membrane remodeling. In addition, it can become activated by binding BCL-2, as well as non-BCL-2 proteins such as p53 and Bif-1. Conversely, BAX can become inactivated by interacting with VDAC2, Pin1, and IBRDC2.

Specifications

Catalog number

ET1603-34

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Apoptosis regulator BAX antibody BAX antibody Bax-protein antibody BAX_HUMAN antibody BAXA antibody Baxdelta2G9 antibody Baxdelta2G9omega antibody Baxdelta2omega antibody Bcl-2-like protein 4 antibody BCL2 associated X protein antibody BCL2 associated X protein omega antibody BCL2 associated X protein transcript variant delta2 antibody Bcl2-L-4 antibody BCL2L4 antibody membrane isoform alpha antibody

Abbreviation

Bcl2-L-4

Swiss Prot

Q07812 Human, Q07813 Mouse, Q63690 Rat

Cellular Locus

Mitochondrion membrane, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Goat

Immunogen

Synthetic peptide within Human Bax aa 1-50 / 192.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, FC

Positive Control

HeLa cell lysate, MCF7 cell lysate, HEK-293 cell lysate, bEnd.3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, C2C12, HeLa.

Concentration

1 mg/mL

Dilution

WB: 1:20,000 ;IF-Cell: 1:100 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 21 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes