Cytokeratin 18 Recombinant Rabbit Monoclonal Antibody [SZ80-07]

About this product

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, Cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while Cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Cytokeratin 18 contains two major phosphorylation sites on Ser 33 and Ser 52. Phosphorylation of Ser 18 is essential for the association of Cytokeratin 18 with 14-3-3 proteins and is involved in keratin organization and distribution.

Specifications

Catalog number

ET1603-8

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cell proliferation inducing gene 46 protein antibody Cell proliferation inducing protein 46 antibody Cell proliferation-inducing gene 46 protein antibody CK 18 antibody CK-18 antibody CK18 antibody CYK 18 antibody CYK18 antibody Cytokeratin 18 antibody Cytokeratin endo B antibody Cytokeratin-18 antibody K 18 antibody K18 antibody K1C18_HUMAN antibody KA18 antibody Keratin 18 antibody Keratin 18, type I antibody Keratin D antibody keratin, type I cytoskeletal 18 antibody Keratin-18 antibody Krt18 antibody

Abbreviation

CK-18, K18, KRT18

Swiss Prot

P05783 Human, P05784 Mouse, Q5BJY9 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within mouse Cytokeratin 18 aa 200-423.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, mIHC

Positive Control

A431 cell lysate, HeLa cell lysate, HT-29 cell lysate, mouse kidney, rat skin tissue, Hela, A431, HepG2, human colon carcinoma tissue, human breast carcinoma tissue, human colon tissue,human liver tissue, human stomach tissue, human stomach carcinoma tissue, MCF-7.

Concentration

1 mg/mL

Dilution

WB: 1:5,000-1:20,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;mIHC: 1:3,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 48 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes