SATB1 Recombinant Rabbit Monoclonal Antibody [SP05-03]

About this product

The homeoproteins CCAAT displacement protein (CDP) and special AT-rich sequence binding protein 1 (SATB1) are transcriptional repressors of many cellular genes, and they participate in cell development and cell type differentiation. SATB1 is expressed primarily in thymocytes, and, like CDP, it also contains a distinct homeobox DNA-binding domain that is essential for DNA binding. SATB1 and CDP interact through these homeodomains and synergistically function as mediators of gene expression. SATB1 contains an additional domain that has a higher affinity for DNA and specifically facilitates the direct association between SATB1 and the nuclear matrix attachment regions (MARs) of DNA. MARs are specific DNA sequences that bind to the nuclear matrix and form the base of chromosomal loops that organize the chromosomes and regulate DNA transcription and replication within the nucleus. The association of SATB1 with the core unwinding element within the base-unpairing region of MARs requires both the MAR and homeobox binding domains of SATB1.

Specifications

Catalog number

ET1604-10

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DNA binding protein SATB1 antibody DNA-binding protein SATB1 antibody SATB homeobox 1 antibody SATB1 antibody SATB1_HUMAN antibody Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody Special AT rich sequence binding protein 1 antibody Special AT-rich sequence-binding protein 1 antibody

Abbreviation

SATB1

Swiss Prot

Q01826 Human, Q60611 Mouse

Cellular Locus

Nucleus matrix, PML body.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human SATB1 aa 5-54 / 763.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Positive Control

Human thymus tissue lysates, mouse thymus tissue lysates, human tonsil tissue, mouse brain tissue, rat spleen tissue, mouse thymus tissue, Jurkat.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 86 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes