FOXO3A Recombinant Rabbit Monoclonal Antibody [SP0054]

About this product

FKHRL1 (forkhead in rhabdomyosarcoma-like 1), also known as FOXO3 (forkheadbox O3) or FOXO3A, is a 673 amino acid transcriptional activator thatbelongs to the FKHR subfamily of forkhead transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 at Threonine 32 and Serine 253. Phosphorylation by Akt1 negatively regulates FKHRL1 by promoting its export from the nucleus. Phosphorylated FKHRL1 associates with 14-3-3 proteins and this complex is retained in the cytoplasm. Growth factor withdrawal stimulates FKHRL1 dephosphorylation and nuclear translocation, leading to FKHR-induced gene-specific transcriptional activation. Within the nucleus, dephosphorylated FKHRL1 triggers apoptosis by inducing the expression of genes that are critical for cell death.

Specifications

Catalog number

ET1604-11

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

AF6q21 antibody AF6q21 protein antibody DKFZp781A0677 antibody FKHR2 antibody FKHRL 1 antibody FKHRL1 antibody FKHRL1P2 antibody Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody Forkhead box O3 antibody Forkhead box O3A antibody Forkhead box protein O3 antibody Forkhead box protein O3A antibody Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody Forkhead homolog (rhabdomyosarcoma) like 1 antibody Forkhead in rhabdomyosarcoma like 1 antibody Forkhead in rhabdomyosarcoma-like 1 antibody FOX O3A antibody FOXO2 antibody foxo3 antibody FOXO3_HUMAN antibody FOXO3A antibody MGC12739 antibody MGC31925 antibody

Abbreviation

FOXO3

Swiss Prot

O43524 Human, Q9WVH4 Mouse,

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human FOXO3A aa 21-69/673.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue

Positive Control

NIH/3T3 cell lysate, MCF-7 cell lysate, PC-12 cell lysate, Hela, MCF-7,SW480.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IF-Cell: 1:100-1:200 ;IF-Tissue: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

71 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes