GFP Recombinant Rabbit Monoclonal Antibody [SP069-0]

About this product

The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.

Specifications

Catalog number

ET1604-26

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

GFP antibody Green fluorescent protein antibody yfp antibody

Abbreviation

GFP

Swiss Prot

P42212 AequoreaVictoria

Host

Rabbit

Species Reactivity

Universal

Immunogen

Recombinant full length protein of Aequorea victoria GFP.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

K562 cell lysates transfected with GFP.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:10,000 ;IF-Cell: 1:500-1:1,000 ;IF-Tissue: 1:500-1:1,000 ;IHC-P: 1:1,000-1:5,000 ;IP: 2-5 µg/ml.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 27 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes