MSH6 Recombinant Rabbit Monoclonal Antibody [SP08-02]

About this product

Multiple pathways promote short-sequence recombination (SSR) in Saccharomyces cerevisiae. When gene conversion is initiated by a double-strand break (DSB), any nonhomologous DNA that may be present at the ends must be removed before new DNA synthesis can be initiated. Removal of a 3' nonhomologous tail in S. cerevisiae depends on the nucleotide excision repair endonuclease Rad1/Rad10 and also on the mismatch repair proteins Msh2 and Msh3. Msh2 and Msh3, which function in mitotic recombination, recognize not only heteroduplex loops and mismatched basepairs, but also branched DNA structures with a free 3' tail. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication. Yeast Msh2-Msh6 interact asymmetrically with the DNA through base-specific stacking and hydrogen bonding interactions and backbone contacts. The importance of these contacts decreases with increasing distance from the mismatch, implying that interactions at or near the mismatch are important for binding in a kinked DNA conformation.

Specifications

Catalog number

ET1604-39

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DNA mismatch repair protein Msh6 antibody G/T mismatch binding protein antibody G/T mismatch-binding protein antibody GTBP antibody GTMBP antibody hMSH6 antibody HNPCC 5 antibody HNPCC5 antibody HSAP antibody MSH 6 antibody MSH6 antibody MSH6_HUMAN antibody mutS (E. coli) homolog 6 antibody MutS alpha 160 kDa subunit antibody MutS homolog 6 (E. coli) antibody mutS homolog 6 antibody MutS-alpha 160 kDa subunit antibody p160 antibody Sperm associated protein antibody

Abbreviation

hMSH6, GTBP, GTMBP, p160

Swiss Prot

P52701 Human, P54276 Mouse

Cellular Locus

Nucleus, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human MSH6 aa 1-50 / 1,360.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

A549 cell lysates, A549, RAW264.7, human tonsil tissue, mouse epididymis tissue, mouse testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:100 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;IP: 1-2μg/sample

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 153 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes