PHD1/prolyl hydroxylase Recombinant Rabbit Monoclonal Antibody [SP00-48]

About this product

Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2 and HIF PHD3 (known as PHD1, PHD2 and PHD3 in rodents, respectively) can hydroxylate HIF-α subunits. Hypoxia-inducible factor (HIF) is a transcriptional regulator important in several aspects of oxygen homeostasis. The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-α proteins. HIF PHD1, which is widely expressed, with highest levels of expression in testis, functions as a cellular oxygen sensor and is important in cell growth regulation. HIF PHD1 can localize to the nucleus or the cytoplasm and is also detected in hormone responsive tissues, such as normal and cancerous mammary, ovarian and prostate epithelium. HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13.3. HIF PHD2 is regarded as the main cellular oxygen sensor, as RNA interference against HIF PHD2, but not HIF PHD1 or HIF PHD3, is enough to stabilize HIF-1α in normoxia. HIF PHD2, a direct HIF target gene, is expressed mainly in skeletal muscle, heart, kidney and brain. HIF PHD3 may play a role in the regulation of cell growth in muscle cells and in apoptosis in neuronal tissue. HIF PHD3 is widely expressed, although the highest levels can be detected in placenta and he

Specifications

Catalog number

ET1604-5

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DKFZp434E026 antibody EGL nine (C.elegans) homolog 2 antibody Egl nine homolog 2 (C. elegans) antibody Egl nine homolog 2 antibody EGLN 2 antibody EGLN2 antibody EGLN2_HUMAN antibody EIT 6 antibody EIT6 antibody Estrogen-induced tag 6 antibody HIF P4H 1 antibody HIF PH1 antibody HIF prolyl hydroxylase 1 antibody HIF-PH1 antibody HIF-prolyl hydroxylase 1 antibody HIFPH 1 antibody HIFPH1 antibody HPH 3 antibody HPH-1 antibody HPH-3 antibody HPH3 antibody Hypoxia inducible factor prolyl hydroxylase 1 antibody Hypoxia-inducible factor prolyl hydroxylase 1 antibody P4H1 antibody PHD 1 antibody PhD1 antibody prolyl hydroxylase domain containing protein 1 antibody Prolyl hydroxylase domain-containing protein 1 antibody

Abbreviation

HIF-PH1, HIF-prolyl hydroxylase 1, HPH-1, PHD1, EGLN2

Swiss Prot

Q96KS0 Human, Q91YE2 Mouse, Q6AYU4 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human PHD1 aa 1-91 / 407.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, Hela, A549, SKOV-3, human lung carcinoma tissue, human breast carcinoma tissue, mouse testis tissue, mouse prostate tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

44 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes