Phospho-Smad5 (S463 + S465) Recombinant Rabbit Monoclonal Antibody [SY09-03]

About this product

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.

Specifications

Catalog number

ET1605-5

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DKFZp781C1895 antibody DKFZp781O1323 antibody Dwfc antibody hSmad5 antibody JV5 1 antibody JV5-1 antibody MAD homolog 5 antibody MAD, mothers against decapentaplegic homolog 5 antibody MADH 5 antibody MADH5 antibody Mothers against decapentaplegic homolog 5 antibody mothers against decapentaplegic, drosophila, homolog of, 5 antibody Mothers against DPP homolog 5 antibody MusMLP antibody SMA and MAD related protein 5 antibody SMAD 5 antibody SMAD family member 5 antibody SMAD, mothers against DPP homolog 5 antibody Smad5 antibody SMAD5_HUMAN antibody

Abbreviation

MAD homolog 5, Mothers against DPP homolog 5, SMAD 5, Smad5, hSmad5, SMAD5, MADH5

Swiss Prot

Q99717 Human, P97454 Mouse, Q9R1V3 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser463 and 465 of Human Smad5. The immunogen may cross with p-smad1/9.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

HeLa treated with 20 ng/mL TGFβ1 for 15 minutes whole cell lysate, Rat brain tissue lysate, mouse brain tissue lysate, HeLa cells treated with 20ng/mL TGFβ1 for 15 minutes, SKOV-3, HepG2, human tonsil tissue, human breast carcinoma tissue, human liver tissue, mouse brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 52 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes