MCL1 Recombinant Rabbit Monoclonal Antibody [SI16-04]

About this product

B-cell CLL/lymphoma 2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia 1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase 3 (GSK3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors, PI 3-kinase and AKT. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice.

Specifications

Catalog number

ET1606-14

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Bcl 2 related protein EAT/mcl1 antibody Bcl-2-like protein 3 antibody Bcl-2-related protein EAT/mcl1 antibody BCL2 related antibody Bcl2-L-3 antibody BCL2L3 antibody EAT antibody Induced myeloid leukemia cell differentiation protein Mcl 1 antibody Induced myeloid leukemia cell differentiation protein Mcl-1 antibody MCL 1 antibody MCL1 antibody MCL1-ES antibody mcl1/EAT antibody MCL1_HUMAN antibody MCL1L antibody MCL1S antibody MGC104264 antibody MGC1839 antibody Myeloid Cell Leukemia 1 antibody Myeloid cell leukemia ES antibody Myeloid cell leukemia sequence 1 antibody Myeloid cell leukemia sequence 1 BCL2 related antibody Myeloid cell leukemia sequence 1 isoform 1 antibody OTTHUMP00000032794 antibody OTTHUMP00000032795 antibody TM antibody

Abbreviation

Bcl2-L-3, MCL1

Swiss Prot

Q07820 Human, P97287 Mouse, Q9Z1P3 Rat

Cellular Locus

Membrane, Cytoplasm, Mitochondrion, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human MCL1 aa 101-150 / 350.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Tissue, IHC-P, IP

Positive Control

Raji cell lysate, Ramos cell lysate, MCF7 cell lysate, SK-OV-3 cell lysate, mouse heart tissue lysates, Hela, HepG2, BT-20, human breast tissue, human kidney tissue, human pancreas tissue, mouse kidney tissue, Jurkat.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 37 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes