Desmin Recombinant Rabbit Monoclonal Antibody [SI18-00]

About this product

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain α-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Desmin makes up attachments between the terminal Z-disc and membrane-associated proteins to form a force-transmitting system. Mutations in the gene encoding for Desmin are associated with adult-onset skeletal myopathy, sporadic disease and mild cardiac involvement.

Specifications

Catalog number

ET1606-30

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

CMD1I antibody CSM1 antibody CSM2 antibody DES antibody DESM_HUMAN antibody Desmin antibody FLJ12025 antibody FLJ39719 antibody FLJ41013 antibody FLJ41793 antibody Intermediate filament protein antibody OTTHUMP00000064865 antibody

Abbreviation

DES

Swiss Prot

P17661 Human, P31001 Mouse, P48675 Rat

Cellular Locus

Cytoplasm, Cell membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Synthetic peptide within Human Desmin aa 421-470 / 470.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, mIHC

Positive Control

C2C12 cell lysate, mouse heart tissue lysate, rat heart tissue lysate, rat skeletal muscle tissue lysates, C2C12, human endometrium tissue, mouse bladder tissue, mouse colon tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:1,000 ;FC: 1:50-1:100 ;mIHC: 1:800

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 54 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes