MMP-2 Recombinant Rabbit Monoclonal Antibody [SI15-04]

About this product

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Activation of MMP-2 requires proteolytic processing. A complex of membrane type 1 MMP (MT1-MMP/MMP14) and tissue inhibitor of metalloproteinase 2 recruits pro-MMP 2 from the extracellular milieu to the cell surface. Activation then requires an active molecule of MT1-MMP and auto catalytic cleavage. Clustering of integrin chains promotes activation of MMP-2. Another factor that will support the activation of MMP-2 is cell-cell clustering. A wild-type activated leukocyte cell adhesion molecule (ALCAM) is also required to activate MMP-2.

Specifications

Catalog number

ET1606-4

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

72 kDa gelatinase antibody 72kD type IV collagenase antibody CLG 4 antibody CLG 4A antibody CLG4 antibody CLG4A antibody Collagenase Type 4 alpha antibody Collagenase type IV A antibody Gelatinase A antibody Gelatinase alpha antibody Gelatinase neutrophil antibody Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody Matrix Metalloproteinase 2 antibody Matrix metalloproteinase II antibody Matrix metalloproteinase-2 antibody MMP 2 antibody MMP II antibody MMP-2 antibody MMP2 antibody MMP2_HUMAN antibody MONA antibody Neutrophil gelatinase antibody PEX antibody TBE 1 antibody TBE-1 antibody

Abbreviation

MMP-2

Swiss Prot

P08253 Human, P33434 Mouse, P33436 Rat

Cellular Locus

Cytoplasm, Nucleus, Membrane, Mitochondrion, Secreted.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human MMP2 aa 101-150 / 660.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

U-87 MG cell lysate, L6 cell lysate, mouse liver tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 74 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes