CD86 Recombinant Rabbit Monoclonal Antibody [SJ20-00]

About this product

Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein constitutively expressed on dendritic cells, Langerhans cells, macrophages, B-cells (including memory B-cells), and on other antigen-presenting cells. Along with CD80, CD86 provides costimulatory signals necessary for T cell activation and survival. Depending on the ligand bound, CD86 can signal for self-regulation and cell-cell association, or for attenuation of regulation and cell-cell disassociation. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. Roles of both CD80 and CD86 are studied in context of many pathologies. Selective inhibition of costimulatory inhibitors was examined in a model of allergic pulmonary inflammation and airway hyper-responsiveness (AHR). Since initial host response to Staphylococcus aureus, especially the immune response based on T cells, is a contributing factor in the pathogenesis of acute pneumonia, role of the CD80/CD86 pathway in pathogenesis was investigated. The costimulatory molecules were also investigated in context of Bronchial Astma, Treg in cancer, and immunotherapy.

Specifications

Catalog number

ET1606-50

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

Activation B7-2 antigen 3 antibody Activation B7-2 antigen antibody B-lymphocyte activation antigen B7-2 2 antibody B-lymphocyte activation antigen B7-2 antibody B7 2 antibody B7 antibody B7-2 antibody B7.2 antibody B70 antibody B72 antigen antibody BU63 antibody CD28 antigen ligand 2 2 antibody CD28 antigen ligand 2 antibody Cd28l2 antibody CD28LG2 antibody CD86 antibody CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody CD86 antigen antibody CD86 molecule antibody CD86_HUMAN antibody CLS1 antibody CTLA-4 counter-receptor B7.2 2, 3 antibody CTLA-4 counter-receptor B7.2 antibody Early T-cell costimulatory molecule 1 antibody ETC-1 antibody FUN 1 antibody FUN-1 antibody LAB72 antibody Ly-58 antibody Ly58 antibody MB7 antibody MB7-2 antibody Membrane glycoprotein antibody MGC34413 antibody T-lymphocyte activation antigen CD86 antibody TS/A-2 antibody

Abbreviation

CD86, CD28LG2

Swiss Prot

P42081 Human, P42082 Mouse,

Cellular Locus

Cell membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human CD86 aa 1-50 / 329.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IP

Positive Control

Daudi cell lysate, Raji cell lysate, Ramos cell lysate, K-562 cell lysate, Mouse lung tissue lysate, Rat lung tissue lysate, Raji.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:500 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 38 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes