SUMO-1 Recombinant Rabbit Monoclonal Antibody [SJ20-03]

About this product

The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2 and SUMO-3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include IkBa, MDM2, p53, PML and Ran GAP1. SUMO-2 and SUMO-3 contribute to a greater percentage of protein modification than does SUMO-1, and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates b-Amyloid generation and may be critical in the onset or progression of Alzheimer’s disease.

Specifications

Catalog number

ET1606-53

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DAP1 antibody GAP modifying protein 1 antibody GAP-modifying protein 1 antibody GMP 1 antibody GMP1 antibody OFC10 antibody PIC 1 antibody PIC1 antibody SENP2 antibody Sentrin 1 antibody Sentrin antibody Small ubiquitin related modifier 1 antibody Small ubiquitin-like modifier 1 antibody Small ubiquitin-related modifier 1 antibody SMT3 antibody SMT3 homolog 3 antibody SMT3 suppressor of mif two 3 homolog 1 antibody SMT3, yeast, homolog 3 antibody Smt3C antibody SMT3H3 antibody SUMO-1 antibody SUMO1 antibody SUMO1_HUMAN antibody Ubiquitin homology domain protein PIC1 antibody Ubiquitin Like 1 antibody Ubiquitin like protein SMT3C antibody Ubiquitin like protein UBL1 antibody Ubiquitin-homology domain protein PIC1 antibody Ubiquitin-like protein SMT3C antibody Ubiquitin-like protein UBL1 antibody UBL 1 antibody UBL1 antibody

Abbreviation

SUMO-1, GMP1, Smt3C, SUMO1, SMT3C, SMT3H3, UBL1

Swiss Prot

P63165 Human, P63166 Mouse, Q5I0H3 Rat

Cellular Locus

Nucleus membrane, Cytoplasm, Nucleus, Cell membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Human SUMO-1 aa 51-100 / 101.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC, ChIP

Positive Control

HeLa cell lysate, A431 cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, A431, NIH/3T3, human thyroid tissue, mouse trachea tissue, mouse esophagus tissue, mouse testis tissue, rat testis tissue, rat esophagus tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:500 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration. ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 12 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes