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Product details

alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY25-03]

Catalog number ET1607-43

Price for ET1607-43

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Size / count 100 µL

About this product

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas –β and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Specifications

Catalog number
ET1607-43
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
alpha SMA a-SMA antibody asma antibody a actin antibody AAT6 antibody ACTA_HUMAN antibody ACTA2 antibody Actin alpha 2 smooth muscle aorta antibody Actin aortic smooth muscle antibody Actin, aortic smooth muscle antibody ACTSA antibody ACTVS antibody Alpha 2 actin antibody Alpha actin 2 antibody Alpha cardiac actin antibody Alpha-actin-2 antibody Cell growth inhibiting gene 46 protein antibody Cell growth-inhibiting gene 46 protein antibody GIG46 antibody Growth inhibiting gene 46 antibody MYMY5 antibody
Abbreviation
ACTA2
Swiss Prot
P62736 Human, P62737 Mouse, P62738 Rat
Cellular Locus
Cytoplasm.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide within N-terminal human alpha smooth muscle Actin.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P, FC, IP
Positive Control
HeLa cell lysate, A431 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, L6 cell lysate, mouse colon tissue lysate, rat colon tissue lysate, human tonsil tissue, human lung tissue, human liver tissue, mouse skin tissue, mouse small intestine tissue, Hela.
Concentration
1 mg/mL
Dilution
WB: 1:2,000-1:5,000;IHC-P:1:50-1:200;FC: 1:50-1:100;IP: Use at an assay dependent concentration.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 42 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY25-03]?

The catalog number for alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY25-03] is ET1607-43.

What sizes are available for alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY25-03]?

alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY25-03] is available in the following size(s): 100 µL.

Is alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY25-03] in stock?

alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY25-03] is currently made to order / not held in immediate stock. Contact us for lead time.

What is alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY25-03] used for?

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only on

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

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