CDK1 Recombinant Rabbit Monoclonal Antibody [SY26-02]

About this product

Cdk1 is a small protein (approximately 34 kilodaltons), and is highly conserved. Cdk1 is comprised mostly by the bare protein kinase motif, which other protein kinases share. Cdk1, like other kinases, contains a cleft in which ATP fits. When bound to its cyclin partners, Cdk1 phosphorylation leads to cell cycle progression. Given its essential role in cell cycle progression, Cdk1 is highly regulated. Most obviously, Cdk1 is regulated by its binding with its cyclin partners. Cyclin binding alters access to the active site of Cdk1, allowing for Cdk1 activity; furthermore, cyclins impart specificity to Cdk1 activity. At least some cyclins contain a hydrophobic patch which may directly interact with substrates, conferring target specificity. Furthermore, cyclins can target Cdk1 to particular subcellular locations.

Specifications

Catalog number

ET1607-51

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cdc 2 antibody Cdc2 antibody CDC28A antibody CDK 1 antibody CDK1 antibody CDK1_HUMAN antibody CDKN1 antibody CELL CYCLE CONTROLLER CDC2 antibody Cell division control protein 2 antibody Cell division control protein 2 homolog antibody Cell division cycle 2 G1 to S and G2 to M antibody Cell division protein kinase 1 antibody Cell Divsion Cycle 2 Protein antibody Cyclin Dependent Kinase 1 antibody Cyclin-dependent kinase 1 antibody DKFZp686L20222 antibody MGC111195 antibody p34 Cdk1 antibody p34 protein kinase antibody P34CDC2 antibody

Abbreviation

CDK1

Swiss Prot

P06493 Human, P11440 Mouse, P39951 Rat

Cellular Locus

Cytoplasm, Nucleus, Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human CDK1 aa 1-50 / 297.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

HeLa cell lysate, Saos-2 cell lysate, Jurkat cell lysate, Mouse spleen tissue lysate, NIH/3T3 cell lysate, C2C12 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, HeLa, human breast cancer tissue, human tonsil tissue, human testis tissue, mouse testis tissue, rat testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 34 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes