alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY02-64]

About this product

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Specifications

Catalog number

ET1607-53

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

alpha SMA a-SMA antibody asma antibody a actin antibody AAT6 antibody ACTA_HUMAN antibody ACTA2 antibody Actin alpha 2 smooth muscle aorta antibody Actin aortic smooth muscle antibody Actin, aortic smooth muscle antibody ACTSA antibody ACTVS antibody Alpha 2 actin antibody Alpha actin 2 antibody Alpha cardiac actin antibody Alpha-actin-2 antibody Cell growth inhibiting gene 46 protein antibody Cell growth-inhibiting gene 46 protein antibody GIG46 antibody Growth inhibiting gene 46 antibody MYMY5 antibody

Abbreviation

ACTA2

Swiss Prot

P62736 Human, P62737 Mouse, P62738 Rat

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within N-terminal human alpha smooth muscle Actin.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, mIHC

Positive Control

Saos-2 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Neuro-2a cell lysate, mouse skin tissue lysate, rat skin tissue lysate, human breast carcinoma tissue, HeLa cell lysate, A431 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, L6 cell lysate, mouse heart tissue lysate, mouse skin tissue lysate, rat heart tissue lysate, rat skin tissue lysate, rat smooth muscle tissue lysate, human kidney tissue, human stomach cancer tissue, human pancreatic carcinoma, HepG2, human tonsil tissue, human liver tissue, human gastric cancer, Human non-small cell lung cancer, mouse pancreas, mouse liver, mouse kidney, human stomach cancer tissue, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue, NIH/3T3, PC-12, HepG2.

Concentration

1 mg/mL

Dilution

WB: 1:5,000-1:50,000 ;IF-Cell: 1:2,000 ;IF-Tissue: 1:1,000 ;IHC-P: 1:50,000 ;FC: 1:1,000-1:2,000 ;mIHC: 1:2,000-1:10,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 42 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes