Phospho-GSK3 (alpha + beta) (Y216 + Y279) Recombinant Rabbit Monoclonal Antibody [SY26-05]

About this product

Glycogen synthase kinase-3α (GSK-3α) and GSK-3β are highly similar isoforms of serine/ threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3β activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser 21 and Ser 9, respectively, through a phosphatidylinositol 3-kinase (PI 3-K)-protein kinase B (PKB)-dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser 9 of GSK-3β and inactivates both forms. GSK-3α/β is positively regulated by phosphorylation on Tyr 279 and Tyr 216, respectively. Activated GSK-3α/β participates in energy metabolism, neuronal cell development, and body pattern formation. Tyrosine dephosphorylation of GSK-3 is involved in its extracellular signal-dependent inactivation.

Specifications

Catalog number

ET1607-54

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Factor A antibody Glycogen synthase kinase 3 alpha antibody Glycogen synthase kinase 3 beta antibody GSK3 alpha antibody GSK3 beta antibody GSK3B antibody

Abbreviation

GSK-3 alpha, GSK3A, GSK-3 beta, GSK3B

Swiss Prot

P49840 Human, P49841 Human, Q2NL51 Mouse, Q9WV60 Mouse, P18265 Rat, P18266 Rat

Cellular Locus

Cytoplasm, Nucleus, Cell membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Tyr216 and 279 of human GSK3 (alpha+beta).

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, IP, IF-Tissue, FC

Positive Control

293T cell lysate, SH-SY5Y cell lysate, HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse cerebellum tissue lysates, A431, Hela, MCF-7, rat brain tissue, rat hippocampus tissue, mouse brain tissue, human thyroid carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IF-Cell: 1:100-1:500 ;IHC-P: 1:200-1:800 ;IP: Use at an assay dependent concentration. ;IF-Tissue:1:200 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 51 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes