Topoisomerase II alpha Recombinant Rabbit Monoclonal Antibody [SY27-00]

About this product

DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination, and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIα and Topo IIβ. Topo IIβ and Topo IIα, are largely homologous at their N-terminal three quarters, however, the C-terminal segments are considerably divergent, suggesting that these regions may mediate different cellular functions and account for the observed differential tissue expression patterns of the two isoforms.

Specifications

Catalog number

ET1607-59

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

alpha isozyme antibody ATP hydrolyzing DNA topoisomerase II alfa antibody DNA gyrase antibody DNA topoisomerase (ATP hydrolyzing) antibody DNA topoisomerase 2 alpha antibody DNA topoisomerase 2-alpha antibody DNA topoisomerase II 170 kD antibody DNA topoisomerase II alpha isozyme antibody DNA topoisomerase II antibody DNA Topoisomerase2 antibody TOP 2A antibody TOP2 antibody TOP2A antibody TOP2A_HUMAN antibody Topoisomerase DNA II alpha 170kDa antibody TP2A antibody Topoisomerase Ⅱ alpha Topoisomerase Ⅱ alpha

Abbreviation

TOP2A, TOP2

Swiss Prot

P11388 Human, Q01320 Mouse, P41516 Rat

Cellular Locus

Nucleoplasm, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Topoisomerase Ⅱ alphaaa 1,482-1,531 / 1,531.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IP

Positive Control

Rat testis tissue lysate, Mouse testis tissue lysate, NIH/3T3 cell lysate, HT-29 cell lysate, Jurkat cell lysate, human testis tissue, mouse testis tissue, rat testis tissue, human tonsil tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:200-1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 174 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes