E-Cadherin Recombinant Rabbit Monoclonal Antibody [SY0287]

About this product

Cadherin-1 (not to be confused with the APC/C activator protein CDH1) also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin is a protein that in humans is encoded by the CDH1 gene. Mutations are correlated with gastric, breast, colorectal, thyroid, and ovarian cancers. CDH1 has also been designated as CD324 (cluster of differentiation 324). It is a tumor suppressor gene. E-cadherin (epithelial) is the most well-studied member of the cadherin family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and, therefore, to E-cadherin function. In epithelial cells, E-cadherin-containing cell-to-cell junctions are often adjacent to actin-containing filaments of the cytoskeleton. E-cadherin is first expressed in the 2-cell stage of mammalian development, and becomes phosphorylated by the 8-cell stage, where it causes compaction.

Specifications

Catalog number

ET1607-75

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Arc 1 antibody CADH1_HUMAN antibody Cadherin 1 antibody cadherin 1 type 1 E-cadherin antibody Cadherin1 antibody CAM 120/80 antibody CD 324 antibody CD324 antibody CD324 antigen antibody cdh1 antibody CDHE antibody E-Cad/CTF3 antibody E Cadherin antibody E-cadherin antibody ECAD antibody Epithelial cadherin antibody epithelial calcium dependant adhesion protein antibody LCAM antibody Liver cell adhesion molecule antibody UVO antibody Uvomorulin antibody

Abbreviation

E-cadherin, CDH1

Swiss Prot

P12830 Human

Cellular Locus

Cell junction, Cell membrane, Endosome, Golgi apparatus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within human E-Cadherin aa 580-630 (Extracellular).

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IP

Positive Control

MCF7 cell lysate, HT-29 cell lysate, HCT 116 cell lysate, A431 cell lysate, Caco-2 cell lysate, HT-29, human lung carcinoma tissue, human colon tissue, human breast cancer tissue.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:2,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 97 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes