Cleaved PARP Recombinant Rabbit Monoclonal Antibody [SU0314]

About this product

Poly (ADP-ribose) polymerase (PARP) is a family of proteins involved in a number of cellular processes such as DNA repair, genomic stability, and programmed cell death. The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. Once PARP detects a SSB, it binds to the DNA, undergoes a structural change, and begins the synthesis of a polymeric adenosine diphosphate ribose (poly (ADP-ribose) or PAR) chain, which acts as a signal for the other DNA-repairing enzymes. Target enzymes include DNA ligase III (LigIII), DNA polymerase beta (polβ), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly (ADP-ribose) glycohydrolase (PARG). PARP enzymes are essential in a number of cellular functions, including expression of inflammatory genes: PARP1 is required for the induction of ICAM-1 gene expression by cardiac myocytes and smooth muscle cells, in response to TNF.

Specifications

Catalog number

ET1608-10

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ADP-ribosyltransferase diphtheria toxin-like 1 antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody APOPAIN antibody ARTD1 antibody NAD (+) ADP-ribosyltransferase 1 antibody PARP antibody PARP-1 antibody PARP1 antibody PARP1_HUMAN antibody Poly [ADP-ribose] polymerase 1 antibody Poly ADP ribose polymerase 1 antibody Poly[ADP-ribose] synthase 1 antibody PPOL antibody SCA1 antibody

Abbreviation

PARP-1, ARTD1, ADPRT 1, PARP1

Swiss Prot

P09874 Human

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human PARP1 aa 200-249 / 1,014.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IP

Positive Control

Jurkat cell lysate, A549 cell lysate, Hela, Daudi cell lysates.

Concentration

1 mg/mL

Dilution

WB: 1:500 ;IF-Cell: 1:50-1:250 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 89 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes