Phospho-HSF1 (S326) Recombinant Rabbit Monoclonal Antibody [SU31-03]

About this product

Heat shock factor 1 (HSF1) is a protein that in humans is encoded by the HSF1 gene. HSF1 is highly conserved in eukaryotes and is the primary mediator of transcriptional responses to proteotoxic stress with important roles in non-stress regulation such as development and metabolism. The HSF1 protein regulates the heat shock response (HSR) pathway in humans by acting as the major transcription factor for heat shock proteins. The HSR plays a protective role by ensuring proper folding and distribution of proteins within cells. This pathway is induced by not only temperature stress, but also by a variety of other stressors such as hypoxic conditions and exposure to contaminants. HSF1 transactivates genes for many cytoprotective proteins involved in heat shock, DNA damage repair, and metabolism. This illustrates the versatile role of HSF1 in not only the heat shock response, but also in aging and diseases.

Specifications

Catalog number

ET1608-11

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Heat shock factor 1 antibody Heat shock factor protein 1 antibody Heat shock transcription factor 1 antibody HSF 1 antibody hsf1 antibody HSF1_HUMAN antibody HSTF 1 antibody HSTF1 antibody

Abbreviation

HSF 1, HSTF 1, HSF1

Swiss Prot

Q00613 Human

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser326 of Human HSF1 aa 301-350 / 529.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IP

Positive Control

HeLa 42°C heat shocked for 30 minutes cell lysate, Hela, AGS, MCF-7, human breast tissue, human tonsil tissue, human lung tissue, human thyroid tissue, human skin tissue, human pancreas tissue, rat bladder tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:200 ;IP: 1-2μg/sample

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 57 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes