PKC alpha Recombinant Rabbit Monoclonal Antibody [SU31-08]

About this product

Protein kinase C alpha (PKCα) is an enzyme that in humans is encoded by the PRKCA gene. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca2+ handling in myocytes. Protein kinase C-alpha (PKC-α) is a specific member of the protein kinase family. These enzymes are characterized by their ability to add a phosphate group to other proteins, thus changing their function.

Specifications

Catalog number

ET1608-15

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

AAG6 antibody Aging associated gene 6 antibody aPKC antibody KPCA_HUMAN antibody PKC alpha antibody PKC-A antibody PKC-alpha antibody PKCA antibody PRKACA antibody PRKCA antibody Protein Kinase C alpha antibody Protein kinase C alpha type antibody

Abbreviation

PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA

Swiss Prot

P17252 Human, P20444 Mouse, P05696 Rat

Cellular Locus

Cytoplasm, Nucleus, Cell membrane, Mitochondrion membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human PKC alpha aa 560-672 / 672.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IP

Positive Control

293T cell lysate, U-87 MG cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, Hela, MCF-7, CRC, A549, PC-12, human lung tissue, mouse brain tissue, mouse small intestine tissue, mouse heart tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: 1:10-1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 77 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes