FOXO1A Recombinant Rabbit Monoclonal Antibody [SU33-01]

About this product

FKHR (for forkhead in rhabdomyosarcoma) and FKHRL1 are members of the forkhead family of transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 and results in FKHRL1 associating with 14-3-3 proteins and being retained in the cytoplasm. Induction of apoptosis or withdrawal of growth factors stimulates dephosphorylation and nuclear translocation of FKHR proteins, leading to FKHR-induced gene-specific transcriptional activation. FKHR, also designated forkhead box protein O1A (FOXO1), is a ubiquitously expressed protein that shuttles between the cytoplasm and nucleus. Genetic mutations in FKHR genes, including the t (2;13) and t (1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. These translocations result in the fusion of the amino terminus of Pax-3 or Pax-7, including the paired box and homeodomain DNA-binding domains, with the carboxy-terminus of FKHR, which contains a transcriptional activation domain. The Pax-3/FKHR fusion protein appears to function as an oncogenic transcription factor that enhances the activation of normal Pax-3 target genes.

Specifications

Catalog number

ET1608-25

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

FKH 1 antibody FKH1 antibody FKHR antibody Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody Forkhead box O1 antibody Forkhead box protein O1 antibody Forkhead box protein O1A antibody Forkhead in rhabdomyosarcoma antibody Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody FoxO transcription factor antibody foxo1 antibody FOXO1_HUMAN antibody FOXO1A antibody OTTHUMP00000018301 antibody

Abbreviation

FOXO1

Swiss Prot

Q12778 Human, Q9R1E0 Mouse, G3V7R4 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Synthetic peptide within Human FOXO1A aa 301-350 / 655.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

THP-1 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HeLa, NIH/3T3, human tonsil tissue, human breast carcinoma tissue, mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:1,000 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 70 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes