Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) Recombinant Rabbit Monoclonal Antibody [SU03-70]

About this product

Serine/threonine-protein kinase PAK 1 is an enzyme that in humans is encoded by the PAK1 gene. PAK1 is one of six members of the PAK family of serine/threonine kinases which are broadly divided into group I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK6 and PAK5/7). The PAKs are evolutionarily conserved. PAK1 localizes in distinct sub-cellular domains in the cytoplasm and nucleus. PAK1 regulates cytoskeleton remodeling, phenotypic signaling and gene expression, and affects a wide variety of cellular processes such as directional motility, invasion, metastasis, growth, cell cycle progression, angiogenesis. PAK1-signaling dependent cellular functions regulate both physiologic and disease processes, including cancer, as PAK1 is widely overexpressed and hyperstimulated in human cancer, at-large. PAK2 and its cleaved fragment localize in both the cytoplasmic or nuclear compartments. PAK2 signaling modulates apoptosis, endothelial lumen formation, viral pathogenesis, and cancer including, breast, hepatocarcinoma, and gastric and cancer, at-large. PAK3 is preferentially expressed in neuronal cells and involved in synapse formation and plasticity and mental retardation.

Specifications

Catalog number

ET1608-58

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Beta PAK antibody bPAK antibody CDKN1A antibody hPAK3 antibody Mental retardation X linked 30 antibody MRX30 antibody MRX47 antibody Oligophrenin 3 antibody OPHN3 antibody p21 (CDKN1A) activated kinase 3 antibody p21 activated kinase 3 antibody p21 CDKN1A activated kinase 3 antibody P21 protein (Cdc42/Rac) activated kinase 3 antibody PAK-3 antibody PAK3 p21 protein (Cdc42/Rac)-activated kinase 3 antibody PAK3beta antibody Pak65alpha antibody Pak65beta antibody Serine threonine protein kinase PAK 3 antibody Stk4 antibody

Abbreviation

PAK-1, PAK1, PAK-2, PAK2, PAK-3, PAK3

Swiss Prot

O75914 Human, Q13153 Human, Q13177 Human, O88643 Mouse, Q61036 Mouse, Q8CIN4 Mouse, P35465 Rat, Q62829 Rat, Q64303 Rat

Cellular Locus

Cell membrane, ruffle membrane, nucleoplasm, Cytoplasm, focal adhesion, invadopodium, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser144 of Human PAK1 aa 121-150 / 545.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IP

Positive Control

Hela cell lysate, NIH/3T3 cell lysate, SH-SY5Y cell lysate, Hela, NIH/3T3, human liver carcinoma tissue, human colon carcinoma tissue, mouse brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 61 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes