Ubiquitin Recombinant Rabbit Monoclonal Antibody [ST46-03]

About this product

Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. This antibody reacts with ubiquitin, a polypeptide w/ Mr. of approx. 8.5kD. It reacts with physiologically occurring conjugates of ubiquitin and intracellular proteins. Specifically recognizes ubiquitinated cytoplasmic inclusion bodies.

Specifications

Catalog number

ET1609-21

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

FLJ25987 antibody MGC8385 antibody Polyubiquitin B antibody RPS 27A antibody RPS27A antibody UBA 52 antibody UBA 80 antibody UBA52 antibody UBA80 antibody UBB antibody UBB_HUMAN antibody UBC antibody UBCEP 1 antibody UBCEP 2 antibody UBCEP1 antibody UBCEP2 antibody Ubiquitin antibody Ubiquitin B antibody

Abbreviation

Ubb

Swiss Prot

P0CG47 Human, P0CG48 Human, P62979 Human, P62987 Human, P0CG49 Mouse, P62991 Mouse, P0CG51 Rat, Q63429 Rat

Cellular Locus

Cytoplasm, Nucleus, Membrane, Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human Ubiquitin aa 1-50.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

HeLa cell lysate, HeLa treated with 10μM MG-132 for 6 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate, PC-12 cell lysate, HepG2, mouse testis tissue, mouse placenta tissue, mouse brain tissue, human esophagus tissue, 293T cell lysate, HepG2 cell lysate.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IF-Cell: 1:200-1:500 ;IF-Tissue: 1:50-1:2,000 ;IHC-P: 1:200-1:1,0000 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes