TOMM20 Recombinant Rabbit Monoclonal Antibody [ST04-72]

About this product

The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP. Tom22 directly associates with Tom40, the major component of the GIP, and thereby forms a stable interaction between the two core complexes to facilitate the fluid movement of preproteins into the mitochondria. The insertion of Tom40 into the Tom machinery requires the initial binding of Tom40 to Tom20 and leads to the efficient incorporation of Tom40 precursors into preexisting Tom complexes.

Specifications

Catalog number

ET1609-25

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

KIAA0016 antibody MAS20 antibody MGC117367 antibody Mitochondrial 20 kDa outer membrane protein antibody Mitochondrial import receptor subunit TOM20 homolog antibody MOM19 antibody Outer mitochondrial membrane receptor Tom20 antibody TOM20 antibody TOM20_HUMAN antibody TOMM20 antibody Translocase of outer mitochondrial membrane 20 homolog (yeast) antibody Translocase of outer mitochondrial membrane 20 homolog type II antibody

Abbreviation

TOMM20

Swiss Prot

Q15388 Human, Q9DCC8 Mouse, Q62760 Rat

Cellular Locus

Mitochondrion outer membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human TOMM20 aa 1-145 / 145.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Positive Control

HeLa cell lysate, Saos-2 cell lysate, HepG2 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HepG2, MCF7 cell lysate, F9 cell lysate, rat lung tissue lysate, NIH/3T3, human liver carcinoma tissue, human kidney tissue, mouse kidney tissue, mouse small intestine tissue, mouse heart tissue, rat large intestine tissue, human liver tissue, HeLa.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:1,000 ;IHC-P: 1:800 ;IF-Tissue: 1:200 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 16 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes