Hexokinase 1 Recombinant Rabbit Monoclonal Antibody [ST47-05]

About this product

The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the “set point” for insulin secretion.

Specifications

Catalog number

ET1609-28

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

BB404130 antibody Brain form hexokinase antibody dea antibody DrHXK1 antibody EC 2.7.1.1 antibody Glycolytic enzyme antibody HEXOKIN antibody hexokinase I antibody Hexokinase PI antibody Hexokinase type I antibody Hexokinase, tumor isozyme antibody Hexokinase-1 antibody Hexokinase-A antibody HK I antibody HK1 antibody HK1 tb antibody Hk1-s antibody HK1-ta antibody HK1-tb antibody HK1-tc antibody HKD antibody HKI antibody HMSNR antibody HXK1 antibody HXK1_HUMAN antibody im:7148527 antibody mHk1-s antibody wu:fc09d08 antibody wu:fc16e02 antibody wu:fc21e02 antibody wu:fq14b11 antibody zgc:55790 antibody zgc:77618 antibody

Abbreviation

HK I

Swiss Prot

P19367 Human, P17710 Mouse, P05708 Rat

Cellular Locus

Mitochondrion outer membrane, Cytoplasm, Membrane, Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human Hexokinase 1 aa 100-140.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Positive Control

MCF7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Hela cell lysate, 293 cell lysate, HepG2 cell lysate, C2C12, MCF7, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:200 ;IHC-P: 1:1,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 102 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes