Dnmt3a Recombinant Rabbit Monoclonal Antibody [ST04-78]

About this product

Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.

Specifications

Catalog number

ET1609-31

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DNA (cytosine 5) methyltransferase 3 alpha antibody DNA (cytosine 5) methyltransferase 3A antibody DNA (cytosine-5)-methyltransferase 3A antibody DNA cytosine methyltransferase 3A2 antibody DNA methyltransferase 3 alpha antibody DNA methyltransferase 3a antibody DNA methyltransferase HsaIIIA antibody DNA MTase HsaIIIA antibody DNM3A_HUMAN antibody DNMT 3a antibody DNMT antibody Dnmt3a antibody DNMT3A2 antibody M.HsaIIIA antibody MCMT antibody OTTHUMP00000201149 antibody TBRS antibody

Abbreviation

Dnmt3a, DNA MTase HsaIIIA, M.HsaIIIA, DNMT3A

Swiss Prot

Q9Y6K1 Human, O88508 Mouse, Q1LZ53 Rat

Cellular Locus

Cytoplasm, Nucleus, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human Dnmt3a aa 600-640.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Tissue, IHC-P

Positive Control

SH-SY5Y cell lysate, Hela cell lysate, HCT116 cell lysate, mouse testis tissue, rat testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:3,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 102 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes