DGCR8 Recombinant Rabbit Monoclonal Antibody [ST04-79]

About this product

Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs.The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding.Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing.Involved in the silencing of embryonic stem cell self-renewal.

Specifications

Catalog number

ET1609-32

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DGCRK6 antibody C22orf12 antibody D16H22S788E antibody D16Wis2 antibody DGCR 8 antibody Dgcr8 antibody DGCR8 microprocessor complex subunit antibody DGCR8_HUMAN antibody DGCRK 6 antibody DiGeorge syndrome critical region 8 antibody DiGeorge syndrome critical region gene 8 antibody Gy1 antibody Microprocessor complex subunit DGCR8 antibody pasha antibody

Abbreviation

DGCR8, C22orf12, DGCRK6

Swiss Prot

Q8WYQ5 Human, Q9EQM6 Mouse

Cellular Locus

Nucleus, cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human DGCR8 aa 383-773 / 773.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IP

Positive Control

PC12 cell lysate, NIH/3T3 cell lysate, Hela cell lysate, Hela, PC12.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 86 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes