PRMT5 Recombinant Rabbit Monoclonal Antibody [ST51-06]

About this product

The formation of the spliceosome includes the assembly of Sm proteins in an ordered manner onto snRNAs. This process is mediated by the survival of a motor neuron (SMN) protein and is enhanced by modification of specific Arginine residues in the Sm proteins to symmetrical dimethylarginines (sDMAs). sDMA modification of Sm proteins is catalyzed by the methylosome, a complex comprised of the type II methyltransferase PRMT5, also designated JAK-binding protein 1), (JBP1), pICln, and two novel factors. PRMT5 binds the Sm proteins via their Arginine- and Glycine-rich (RG) domains, while pICln binds the Sm domains. PRMT5 is a distinct member of the protein-Arginine methyltransferase (PRMT) family, and predominantly localizes to the cytoplasm in a wide variety of tissues. PRMT5 also associates specifically with the transcription start site region of the cyclin E1 promoter, and, therefore, is involved in the control of transcription and proliferation. The gene encoding human PRMT5 maps to chromosome 14q11.

Specifications

Catalog number

ET1609-43

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

72 kDa ICln binding protein antibody 72 kDa ICln-binding protein antibody ANM5_HUMAN antibody Histone synthetic lethal 7, S. cerevisiae, homolog of antibody Histone-arginine N-methyltransferase PRMT5 antibody HMT1 hnRNP methyltransferase like 5 antibody HOMOLOG OF; SKB1 antibody HRMT1L5 antibody IBP72 antibody Jak-binding protein 1 antibody JBP 1 antibody JBP1 antibody PRMT 5 antibody PRMT5 antibody Protein arginine methyltransferase 5 antibody Protein arginine N methyltransferase 5 antibody Protein arginine N methyltransferase 5 N terminally processed antibody Protein arginine N-methyltransferase 5 antibody S. POMBE antibody S. POMBE HOMOLOG OF; SKB1 antibody SHK1 KINASE BINDING PROTEIN 1 antibody Shk1 kinase binding protein 1 homolog antibody Shk1 kinase-binding protein 1 homolog antibody Shk1 kinase/binding protein 1, S. pombe, homolog of antibody SKB 1 antibody SKB1 antibody SKB1 homolog antibody SKB1: SKB1 homolog (S. pombe) antibody SKB1Hs antibody

Abbreviation

SKB1 homolog, SKB1Hs, HRMT1L5, IBP72, JBP1, SKB1

Swiss Prot

O14744 Human, Q8CIG8 Mouse, D4A0E8 Rat

Cellular Locus

Cytoplasm, Nucleus, Golgi apparatus, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthetic peptide within Human PRMT5 aa 48-91 / 637.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P

Positive Control

HepG2 cell lysate, HEK-293 cell lysate, Raji cell lysate, Jurkat cell lysate, A431 cell lysate, C6 cell lysate, PC-12 cell lysate, bEnd.3 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, HepG2, MCF7 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, Neuro-2a, human kidney tissue, mouse ovary tissue, rat ovary tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:5,000 ;IF-Cell: 1:50-1:250 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:500-1:2,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 73 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes