Phospho-FOXO3a (S253) Recombinant Rabbit Monoclonal Antibody [ST49-01]

About this product

FOXO3 belongs to the O subclass of the forkhead family of transcription factors which are characterized by a distinct fork head DNA-binding domain. There are three other FoxO family members in humans, FOXO1, FOXO4 and FOXO6. These transcription factors share the ability to be inhibited and translocated out of the nucleus on phosphorylation by proteins such as Akt/PKB in the PI3K signaling pathway (aside from FOXO6, which may be constitutively nuclear). Other post-translational modifications including acetylation and methylation are seen and can result in increased or altered FOXO3a activity. The use of FOXO3a knockout mice has revealed a diverse range of functions in both health and disease, namely infertility, lymphoproliferation, adenoma, organ inflammation, metabolism etc.; yet despite the purported importance of FOXO transcription factors in aging, FOXO3A knockout mice do not show an obvious shortening of lifespan or accelerated aging.

Specifications

Catalog number

ET1609-49

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

AF6q21 antibody AF6q21 protein antibody DKFZp781A0677 antibody FKHR2 antibody FKHRL 1 antibody FKHRL1 antibody FKHRL1P2 antibody Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody Forkhead box O3 antibody Forkhead box O3A antibody Forkhead box protein O3 antibody Forkhead box protein O3A antibody Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody Forkhead homolog (rhabdomyosarcoma) like 1 antibody Forkhead in rhabdomyosarcoma like 1 antibody Forkhead in rhabdomyosarcoma-like 1 antibody FOX O3A antibody FOXO2 antibody foxo3 antibody FOXO3_HUMAN antibody FOXO3A antibody MGC12739 antibody MGC31925 antibody

Abbreviation

FOXO3

Swiss Prot

O43524 Human, Q9WVH4 Mouse, D3ZBQ1 Rat

Cellular Locus

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic Human Metabotropic Glutamate Receptor 5 aa 1150 to the C-terminus aa 238-282 / 673.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, FC

Positive Control

Hela cell lysate, PC-12 cell lysate, THP-1 cell lysate, K562 cell lysate, human tonsil tissue, human breast carcinoma tissue, human pancreas tissue, mouse pancreas tissue, 293T whole cell lysate, 293T treated with 50 uM LY294002 and 1 uM Wortmannin for 16 hours whole cell lysate.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:100 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 71 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes