Phospho-MEK1/2 (S218 + S222) Recombinant Rabbit Monoclonal Antibody [ST0490]

About this product

Activation of extracellular signal-regulated kinase (ERK) or mitogen-activated protein kinase by MEK (mitogen-activated protein kinase or extracellular signal-regulated kinase kinase) is an essential event in the mitogenic growth factor-induced signal transduction pathway. Phosphorylation of MEKs correlates with their ability to phosphorylate and activate ERKs. MEK1 and MEK2 can also be activated by autophosphorylation. Lipopolysaccharide activates many of the MAPK family members of the immediate upstream MAPK activator MEK1, MEK2, and MEK3. In plants, MEK can phosphorylate and activate MAPK, and that Tyr phosphorylation is critical for the catalytic activity of MAPK in plants.

Specifications

Catalog number

ET1609-50

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Dual specificity mitogen activated protein kinase kinase 1 antibody Dual specificity mitogen-activated protein kinase kinase 1 antibody ERK activator kinase 1 antibody MAP kinase kinase 1 antibody MAP kinase/Erk kinase 1 antibody MAP2K1 antibody MAPK/ERK kinase 1 antibody MAPKK 1 antibody MAPKK1 antibody MEK 1 antibody Mek1 antibody MEKK1 antibody Mitogen activated protein kinase kinase 1 antibody MKK 1 antibody MKK1 antibody MP2K1_HUMAN antibody PRKMK1 antibody Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody Protein kinase mitogen activated, kinase 1 antibody hide

Abbreviation

MAP kinase kinase 1, MAPKK 1, MKK1, MEK 1, MAP2K1, MEK1, PRKMK1

Swiss Prot

Q02750 Human, P31938 Mouse, Q01986 Rat

Cellular Locus

Cytoplasm, Cytoskeleton, Membrane, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser218 and 222 of Human MEK1 aa 200-249 / 393.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, C6 treated with 200nM PMA for 30 minutes cell lysate, Hela cell lysate, A431 cell lysate, 293T cell lysate, NIH/3T3 cells treated with 200nM PMA for 30 minutes, Hela, HepG2, human tonsil tissue, human liver carcinoma tissue, human spleen tissue, human breast carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 43 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes