Histone H3.3 Recombinant Rabbit Monoclonal Antibody [ST50-08]

About this product

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Specifications

Catalog number

ET1609-59

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

H3 histone family 3A antibody H3 histone family 3B antibody H3 histone, family 3B (H3.3B) antibody H3.3 antibody H3.3A antibody H3.3B antibody H33_HUMAN antibody H3F3 antibody H3F3A antibody H3f3b antibody Histone H3.3 antibody Histone H3.3Q antibody Histone H3.A antibody Histone H3.B antibody MGC87782 antibody MGC87783 antibody

Abbreviation

H3F3B, H3F3A

Swiss Prot

P84243 Human, P84244 Mouse, P84245 Rat

Cellular Locus

Nucleus, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human Histone H3.3 aa 50-100.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, ChIP

Positive Control

HeLa cell lysate, C2C12 cell lysate, C6 cell lysate, BT-20, NIH/3T3, human colon carcinoma tissue, mouse colon tissue, mouse brain tissue, mouse stomach tissue, human lung tissue, human cervix tissue, human skin tissue, human placenta tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:500 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 15 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes