nNOS Recombinant Rabbit Monoclonal Antibody [ST520]

About this product

Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.

Specifications

Catalog number

ET1609-61

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

2310005C01Rik antibody BNOS antibody Constitutive NOS antibody EC 1.14.13.39 antibody IHPS 1 antibody IHPS1 antibody N-NOS antibody NC-NOS antibody neuronal Nitric Oxide Synthase antibody Neuronal NOS antibody Nitric oxide synthase , neuronal, included antibody Nitric oxide synthase 1 (neuronal) antibody Nitric oxide synthase 1 antibody Nitric oxide synthase, brain antibody Nitric oxide synthase, penile neuronal, included antibody NNOS antibody NO antibody NOS 1 antibody NOS antibody NOS type I antibody NOS-I antibody NOS1 antibody NOS1_HUMAN antibody Peptidyl-cysteine S-nitrosylase NOS1 antibody

Abbreviation

N-NOS, nNOS, NOS1

Swiss Prot

P29475 Human, Q9Z0J4 Mouse, P29476 Rat

Cellular Locus

Cell membrane, sarcolemma, Cell projection, dendritic spine.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human nNOS aa 1398-1434 / 1434.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Tissue, IHC-P, IP

Positive Control

Mouse brain tissue lysate, Mouse hippocampus tissue lysate, Mouse cerebellum tissue lysate, Rat hippocampus tissue lysate, Rat cerebellum tissue lysate, human skeletal muscle tissue, rat brain tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 161 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes