Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05]

About this product

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. Cytokeratin 7 (also known as sarcolectin) agglutinates normal and transformed cells with a high affinity for simple sugars. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane.

Specifications

Catalog number

ET1609-62

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CK 7 antibody CK-7 antibody CK7 antibody Cytokeratin 7 antibody Cytokeratin-7 antibody D15Wsu77e antibody K2C7 antibody K2C7_HUMAN antibody K7 antibody Keratin 7 antibody Keratin 7, type II antibody Keratin type II cytoskeletal 7 antibody Keratin, 55K type II cytoskeletal antibody Keratin, simple epithelial antibody Keratin, simple epithelial type I, K7 antibody Keratin, type II cytoskeletal 7 antibody Keratin-7 antibody Krt2-7 antibody KRT7 antibody MGC11625 antibody MGC129731 antibody MGC3625 antibody Sarcolectin antibody SCL antibody Type II mesothelial keratin K7 antibody Type-II keratin Kb7 antibody Cytokeratin7

Abbreviation

CK-7, K7, KRT7

Swiss Prot

P08729 Human, Q9DCV7 Mouse

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human Cytokeratin 7 aa 18-67.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Positive Control

HeLa cell lysate, A549 cell lysate, A549, human breast tissue, human breast carcinoma tissue, SK-Br-3, human liver tissue.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:400 ;IHC-P: 1:1,000-1:2,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 51 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes