Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09] — image 1 of 3

Product details

Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09]

Catalog number ET1609-71

Price for ET1609-71

Size / count 100 µL

About this product

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.

Specifications

Catalog number: ET1609-71
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: C85740 antibody Cell cycle checkpoint kinase antibody Checkpoint , S. pombe, homolog of, 1 antibody Checkpoint kinase 1 antibody Checkpoint kinase 1 homolog (S. pombe) antibody CHEK 1 antibody Chek1 antibody Chk 1 antibody Chk1 antibody CHK1 checkpoint homolog (S. pombe) antibody CHK1_HUMAN antibody EC 2.7.11.1 antibody rad27 antibody Serine/threonine protein kinase Chk1 antibody Serine/threonine-protein kinase CHK1 antibody STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
Abbreviation: CHEK1, CHK1
Swiss Prot: O14757 Human, O35280 Mouse
Cellular Locus: Cytoplasm, Nucleus.
Host: Rabbit
Species Reactivity: Human
Immunogen: Synthetic peptide within Human Chk1 aa 431-476 / 476.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell
Positive Control: Hela cell lysate, Daudi cell lysate, HL-60 cell lysate, HeLa, MCF-7, PC-3M.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 54 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09]?

The catalog number for Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09] is ET1609-71.

What sizes are available for Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09]?

Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09] is available in the following size(s): 100 µL.

Is Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09] in stock?

Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09] used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.